| Children |
| | 1. Henry Frederick STEWART, b. 19 Feb 1594, Stirling Castle, Stirling, Scotland d. 6 Nov 1612, St. James's Palace, London (Age 18 years) |
| + | 2. Elizabeth STEWART, Queen of Bohemia, b. 19 Aug 1596, Falkland Palace, Fife, Scotland d. 13 Feb 1662, Leicester House, London, England (Age 65 years) |
| | 3. Margaret STEWART, b. 24 Dec 1598, Dalkeith Palace, Edinburgh, Scotland d. Mar 1600, Linlithgow, Stirling, Scotland (Age 1 year) |
| + | 4. Charles I STEWART, b. 19 Nov 1600, Dunfermline, Fife, Scotland d. 30 Jan 1649, Whitehall Palace, London (Age 48 years) |
| | 5. Robert STEWART, Duke of Kintyre, b. 18 Feb 1601, Dunfermline, Fife, Scotland d. 27 May 1602, Dunfermline, Fife, Scotland (Age 1 year) |
| | 6. Infant STEWART, b. May 1603, Stirling Castle, Stirling, Scotland d. May 1603, Stirling Castle, Stirling, Scotland (Age 0 years) |
| | 7. Mary STEWART, b. 8 Apr 1605, Greenwich Palace, Kent, England d. 16 Sep 1607, Stanwell Park, Middlesex, England (Age 2 years) |
| | 8. Sophia STEWART, b. 22 Jun 1606, Greenwich Palace, Kent, England d. 23 Jun 1606, Greenwich Palace, Kent, England (Age 0 years) |
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